This project seeks to identify cellular pathways that could be targeted to treat or prevent cancer in FA. The investigators take a novel approach using a high-resolution CRISPR knockout library to screen for fitness genes and FANCA-specific cancer vulnerabilities. They will take advantage of their one-of-a-kind cell lines derived from primary human tumor specimens taken from FA patients. They will also screen for genes that affect oxygen-tolerance, hoping to identify candidate targets for chemoprevention in FANCA-mutant cells. The investigators propose that this state-of-the-art approach will reveal oncogenic drivers specific for tumor cells and that this information can be further exploited to identify drug targets for FA-associated tumors.
Researchers: Josephine Dorsman